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red fluorescent beads  (Thermo Fisher)
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Red Fluorescent Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Red Fluorescent Protein Trap Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a ) Retina flatmount from a mouse injected with AAV2/2-hSyn-GAP43-GCaMP6s into the vitreous of the eye. ( b ) Coronal brain section from the same mouse showing SC with <t>fluorescent</t> RGC axon terminals. ( c ) In vivo 2P fluorescence image of RGC boutons in sSC. ( d ) Example ΔF/F 0 traces and polar plots for 8 regions of interest (ROIs) circled in c , averaged across stimulus repeats. Dashed line: onset of drifting grating. Shaded areas: ± S.E.M. Polar plots: outer circle represents maximal ΔF/F 0 ; inner circle represents 50% of max ΔF/F 0 . ( e ) Direction-selective (DS) boutons color-coded by preferred direction. Gray boutons are visually responsive (max ΔF/F 0 > 10%) but not DS. Bars indicate the grating orientation and arrows indicate direction of motion, respectively. ( f ) Orientation-selective (OS) boutons color-coded by preferred orientation. Gray boutons are visually responsive but not OS. ( g ) DS boutons color-coded by preferred direction in 25 FOVs over 450 μm × 450 μm in sSC. B.V.: blood vessel. ( h ) gDSI distributions for DS (gray bars; 2268 boutons, median 0.43) or non-DS (hollow bars; 4742 boutons, median 0.19) boutons in the FOVs in g . p value: 8.63e-154. ( i ) Preferred direction distribution for DS boutons. ( j ) OS boutons color-coded by preferred orientation for the same FOVs shown in g . ( k ) gOSI distributions for OS (gray bars; 2374 boutons, median 0.33) or non-OS (hollow bars; 4636 boutons, median 0.22) boutons in j . p value: 9.89e-80. ( l ) Preferred orientation distribution for OS boutons.
Diameter Red Fluorescent Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rfp trap beads  (Proteintech)
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( a ) Retina flatmount from a mouse injected with AAV2/2-hSyn-GAP43-GCaMP6s into the vitreous of the eye. ( b ) Coronal brain section from the same mouse showing SC with <t>fluorescent</t> RGC axon terminals. ( c ) In vivo 2P fluorescence image of RGC boutons in sSC. ( d ) Example ΔF/F 0 traces and polar plots for 8 regions of interest (ROIs) circled in c , averaged across stimulus repeats. Dashed line: onset of drifting grating. Shaded areas: ± S.E.M. Polar plots: outer circle represents maximal ΔF/F 0 ; inner circle represents 50% of max ΔF/F 0 . ( e ) Direction-selective (DS) boutons color-coded by preferred direction. Gray boutons are visually responsive (max ΔF/F 0 > 10%) but not DS. Bars indicate the grating orientation and arrows indicate direction of motion, respectively. ( f ) Orientation-selective (OS) boutons color-coded by preferred orientation. Gray boutons are visually responsive but not OS. ( g ) DS boutons color-coded by preferred direction in 25 FOVs over 450 μm × 450 μm in sSC. B.V.: blood vessel. ( h ) gDSI distributions for DS (gray bars; 2268 boutons, median 0.43) or non-DS (hollow bars; 4742 boutons, median 0.19) boutons in the FOVs in g . p value: 8.63e-154. ( i ) Preferred direction distribution for DS boutons. ( j ) OS boutons color-coded by preferred orientation for the same FOVs shown in g . ( k ) gOSI distributions for OS (gray bars; 2374 boutons, median 0.33) or non-OS (hollow bars; 4636 boutons, median 0.22) boutons in j . p value: 9.89e-80. ( l ) Preferred orientation distribution for OS boutons.
Rfp Trap Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent fiducial beads red cat.# f8801  (Thermo Fisher)
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( a ) Retina flatmount from a mouse injected with AAV2/2-hSyn-GAP43-GCaMP6s into the vitreous of the eye. ( b ) Coronal brain section from the same mouse showing SC with <t>fluorescent</t> RGC axon terminals. ( c ) In vivo 2P fluorescence image of RGC boutons in sSC. ( d ) Example ΔF/F 0 traces and polar plots for 8 regions of interest (ROIs) circled in c , averaged across stimulus repeats. Dashed line: onset of drifting grating. Shaded areas: ± S.E.M. Polar plots: outer circle represents maximal ΔF/F 0 ; inner circle represents 50% of max ΔF/F 0 . ( e ) Direction-selective (DS) boutons color-coded by preferred direction. Gray boutons are visually responsive (max ΔF/F 0 > 10%) but not DS. Bars indicate the grating orientation and arrows indicate direction of motion, respectively. ( f ) Orientation-selective (OS) boutons color-coded by preferred orientation. Gray boutons are visually responsive but not OS. ( g ) DS boutons color-coded by preferred direction in 25 FOVs over 450 μm × 450 μm in sSC. B.V.: blood vessel. ( h ) gDSI distributions for DS (gray bars; 2268 boutons, median 0.43) or non-DS (hollow bars; 4742 boutons, median 0.19) boutons in the FOVs in g . p value: 8.63e-154. ( i ) Preferred direction distribution for DS boutons. ( j ) OS boutons color-coded by preferred orientation for the same FOVs shown in g . ( k ) gOSI distributions for OS (gray bars; 2374 boutons, median 0.33) or non-OS (hollow bars; 4636 boutons, median 0.22) boutons in j . p value: 9.89e-80. ( l ) Preferred orientation distribution for OS boutons.
Fluorescent Fiducial Beads Red Cat.# F8801, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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red fluorescence beads  (Thermo Fisher)
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( a ) Retina flatmount from a mouse injected with AAV2/2-hSyn-GAP43-GCaMP6s into the vitreous of the eye. ( b ) Coronal brain section from the same mouse showing SC with <t>fluorescent</t> RGC axon terminals. ( c ) In vivo 2P fluorescence image of RGC boutons in sSC. ( d ) Example ΔF/F 0 traces and polar plots for 8 regions of interest (ROIs) circled in c , averaged across stimulus repeats. Dashed line: onset of drifting grating. Shaded areas: ± S.E.M. Polar plots: outer circle represents maximal ΔF/F 0 ; inner circle represents 50% of max ΔF/F 0 . ( e ) Direction-selective (DS) boutons color-coded by preferred direction. Gray boutons are visually responsive (max ΔF/F 0 > 10%) but not DS. Bars indicate the grating orientation and arrows indicate direction of motion, respectively. ( f ) Orientation-selective (OS) boutons color-coded by preferred orientation. Gray boutons are visually responsive but not OS. ( g ) DS boutons color-coded by preferred direction in 25 FOVs over 450 μm × 450 μm in sSC. B.V.: blood vessel. ( h ) gDSI distributions for DS (gray bars; 2268 boutons, median 0.43) or non-DS (hollow bars; 4742 boutons, median 0.19) boutons in the FOVs in g . p value: 8.63e-154. ( i ) Preferred direction distribution for DS boutons. ( j ) OS boutons color-coded by preferred orientation for the same FOVs shown in g . ( k ) gOSI distributions for OS (gray bars; 2374 boutons, median 0.33) or non-OS (hollow bars; 4636 boutons, median 0.22) boutons in j . p value: 9.89e-80. ( l ) Preferred orientation distribution for OS boutons.
Red Fluorescence Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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red fluorescence beads t8870  (Thermo Fisher)
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( a ) Retina flatmount from a mouse injected with AAV2/2-hSyn-GAP43-GCaMP6s into the vitreous of the eye. ( b ) Coronal brain section from the same mouse showing SC with <t>fluorescent</t> RGC axon terminals. ( c ) In vivo 2P fluorescence image of RGC boutons in sSC. ( d ) Example ΔF/F 0 traces and polar plots for 8 regions of interest (ROIs) circled in c , averaged across stimulus repeats. Dashed line: onset of drifting grating. Shaded areas: ± S.E.M. Polar plots: outer circle represents maximal ΔF/F 0 ; inner circle represents 50% of max ΔF/F 0 . ( e ) Direction-selective (DS) boutons color-coded by preferred direction. Gray boutons are visually responsive (max ΔF/F 0 > 10%) but not DS. Bars indicate the grating orientation and arrows indicate direction of motion, respectively. ( f ) Orientation-selective (OS) boutons color-coded by preferred orientation. Gray boutons are visually responsive but not OS. ( g ) DS boutons color-coded by preferred direction in 25 FOVs over 450 μm × 450 μm in sSC. B.V.: blood vessel. ( h ) gDSI distributions for DS (gray bars; 2268 boutons, median 0.43) or non-DS (hollow bars; 4742 boutons, median 0.19) boutons in the FOVs in g . p value: 8.63e-154. ( i ) Preferred direction distribution for DS boutons. ( j ) OS boutons color-coded by preferred orientation for the same FOVs shown in g . ( k ) gOSI distributions for OS (gray bars; 2374 boutons, median 0.33) or non-OS (hollow bars; 4636 boutons, median 0.22) boutons in j . p value: 9.89e-80. ( l ) Preferred orientation distribution for OS boutons.
Red Fluorescence Beads T8870, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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red-fluorescent beads phrodo green p35369  (Thermo Fisher)
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( a ) Retina flatmount from a mouse injected with AAV2/2-hSyn-GAP43-GCaMP6s into the vitreous of the eye. ( b ) Coronal brain section from the same mouse showing SC with <t>fluorescent</t> RGC axon terminals. ( c ) In vivo 2P fluorescence image of RGC boutons in sSC. ( d ) Example ΔF/F 0 traces and polar plots for 8 regions of interest (ROIs) circled in c , averaged across stimulus repeats. Dashed line: onset of drifting grating. Shaded areas: ± S.E.M. Polar plots: outer circle represents maximal ΔF/F 0 ; inner circle represents 50% of max ΔF/F 0 . ( e ) Direction-selective (DS) boutons color-coded by preferred direction. Gray boutons are visually responsive (max ΔF/F 0 > 10%) but not DS. Bars indicate the grating orientation and arrows indicate direction of motion, respectively. ( f ) Orientation-selective (OS) boutons color-coded by preferred orientation. Gray boutons are visually responsive but not OS. ( g ) DS boutons color-coded by preferred direction in 25 FOVs over 450 μm × 450 μm in sSC. B.V.: blood vessel. ( h ) gDSI distributions for DS (gray bars; 2268 boutons, median 0.43) or non-DS (hollow bars; 4742 boutons, median 0.19) boutons in the FOVs in g . p value: 8.63e-154. ( i ) Preferred direction distribution for DS boutons. ( j ) OS boutons color-coded by preferred orientation for the same FOVs shown in g . ( k ) gOSI distributions for OS (gray bars; 2374 boutons, median 0.33) or non-OS (hollow bars; 4636 boutons, median 0.22) boutons in j . p value: 9.89e-80. ( l ) Preferred orientation distribution for OS boutons.
Red Fluorescent Beads Phrodo Green P35369, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a ) Retina flatmount from a mouse injected with AAV2/2-hSyn-GAP43-GCaMP6s into the vitreous of the eye. ( b ) Coronal brain section from the same mouse showing SC with <t>fluorescent</t> RGC axon terminals. ( c ) In vivo 2P fluorescence image of RGC boutons in sSC. ( d ) Example ΔF/F 0 traces and polar plots for 8 regions of interest (ROIs) circled in c , averaged across stimulus repeats. Dashed line: onset of drifting grating. Shaded areas: ± S.E.M. Polar plots: outer circle represents maximal ΔF/F 0 ; inner circle represents 50% of max ΔF/F 0 . ( e ) Direction-selective (DS) boutons color-coded by preferred direction. Gray boutons are visually responsive (max ΔF/F 0 > 10%) but not DS. Bars indicate the grating orientation and arrows indicate direction of motion, respectively. ( f ) Orientation-selective (OS) boutons color-coded by preferred orientation. Gray boutons are visually responsive but not OS. ( g ) DS boutons color-coded by preferred direction in 25 FOVs over 450 μm × 450 μm in sSC. B.V.: blood vessel. ( h ) gDSI distributions for DS (gray bars; 2268 boutons, median 0.43) or non-DS (hollow bars; 4742 boutons, median 0.19) boutons in the FOVs in g . p value: 8.63e-154. ( i ) Preferred direction distribution for DS boutons. ( j ) OS boutons color-coded by preferred orientation for the same FOVs shown in g . ( k ) gOSI distributions for OS (gray bars; 2374 boutons, median 0.33) or non-OS (hollow bars; 4636 boutons, median 0.22) boutons in j . p value: 9.89e-80. ( l ) Preferred orientation distribution for OS boutons.
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Image Search Results


( a ) Retina flatmount from a mouse injected with AAV2/2-hSyn-GAP43-GCaMP6s into the vitreous of the eye. ( b ) Coronal brain section from the same mouse showing SC with fluorescent RGC axon terminals. ( c ) In vivo 2P fluorescence image of RGC boutons in sSC. ( d ) Example ΔF/F 0 traces and polar plots for 8 regions of interest (ROIs) circled in c , averaged across stimulus repeats. Dashed line: onset of drifting grating. Shaded areas: ± S.E.M. Polar plots: outer circle represents maximal ΔF/F 0 ; inner circle represents 50% of max ΔF/F 0 . ( e ) Direction-selective (DS) boutons color-coded by preferred direction. Gray boutons are visually responsive (max ΔF/F 0 > 10%) but not DS. Bars indicate the grating orientation and arrows indicate direction of motion, respectively. ( f ) Orientation-selective (OS) boutons color-coded by preferred orientation. Gray boutons are visually responsive but not OS. ( g ) DS boutons color-coded by preferred direction in 25 FOVs over 450 μm × 450 μm in sSC. B.V.: blood vessel. ( h ) gDSI distributions for DS (gray bars; 2268 boutons, median 0.43) or non-DS (hollow bars; 4742 boutons, median 0.19) boutons in the FOVs in g . p value: 8.63e-154. ( i ) Preferred direction distribution for DS boutons. ( j ) OS boutons color-coded by preferred orientation for the same FOVs shown in g . ( k ) gOSI distributions for OS (gray bars; 2374 boutons, median 0.33) or non-OS (hollow bars; 4636 boutons, median 0.22) boutons in j . p value: 9.89e-80. ( l ) Preferred orientation distribution for OS boutons.

Journal: bioRxiv

Article Title: Retinal ganglion cell input to superior colliculus encodes salient information

doi: 10.1101/2025.11.06.687035

Figure Lengend Snippet: ( a ) Retina flatmount from a mouse injected with AAV2/2-hSyn-GAP43-GCaMP6s into the vitreous of the eye. ( b ) Coronal brain section from the same mouse showing SC with fluorescent RGC axon terminals. ( c ) In vivo 2P fluorescence image of RGC boutons in sSC. ( d ) Example ΔF/F 0 traces and polar plots for 8 regions of interest (ROIs) circled in c , averaged across stimulus repeats. Dashed line: onset of drifting grating. Shaded areas: ± S.E.M. Polar plots: outer circle represents maximal ΔF/F 0 ; inner circle represents 50% of max ΔF/F 0 . ( e ) Direction-selective (DS) boutons color-coded by preferred direction. Gray boutons are visually responsive (max ΔF/F 0 > 10%) but not DS. Bars indicate the grating orientation and arrows indicate direction of motion, respectively. ( f ) Orientation-selective (OS) boutons color-coded by preferred orientation. Gray boutons are visually responsive but not OS. ( g ) DS boutons color-coded by preferred direction in 25 FOVs over 450 μm × 450 μm in sSC. B.V.: blood vessel. ( h ) gDSI distributions for DS (gray bars; 2268 boutons, median 0.43) or non-DS (hollow bars; 4742 boutons, median 0.19) boutons in the FOVs in g . p value: 8.63e-154. ( i ) Preferred direction distribution for DS boutons. ( j ) OS boutons color-coded by preferred orientation for the same FOVs shown in g . ( k ) gOSI distributions for OS (gray bars; 2374 boutons, median 0.33) or non-OS (hollow bars; 4636 boutons, median 0.22) boutons in j . p value: 9.89e-80. ( l ) Preferred orientation distribution for OS boutons.

Article Snippet: Before placing the cranial window onto the mouse, 5 μL of solution containing 2 μm diameter red fluorescent beads (Invitrogen FluoSpheres; diluted in phosphate buffered saline) were pipetted onto the surface of the SC to aid in adaptive optics correction of aberrations.

Techniques: Injection, In Vivo, Fluorescence